Biosynthesis of ZnO NPs from pumpkin seeds’ extract and elucidation of its anticancer potential against breast cancer

Abstract The cost-effective and environmental friendly biosynthesis of metal nanoparticles is becoming increasingly important in biomedical science. This study biosynthesized zinc oxide (ZnO NPs) from pumpkin seed extract assessed its anticancer activity by using different molecular biology experiments human breast cancer (MDA-MB-231) cell lines. well-known analytical techniques, such as UV-visible spectrophotometry, energy-dispersive X-ray spectroscopy, field emission scanning electron microscopy, transmission microscopy (TEM), were used to confirm the synthesis characterize morphological structural features biogenic ZnO NPs. formation spherical with an average size 50–60 nm was recorded TEM. We observed dose-dependent increase cytotoxicity NPs MDA-MB-231 line a 50% inhibitory concentration 10 µg/mL. Moreover, also showed significant morphology changes, apoptosis induction, reactive oxygen species production at highest tested concentration. cellular adhesion migration assay indicated death inhibition cells response NPs’ treatment. These results clearly demonstrated potential against studied line. However, extension this recommended models and, based on results, vivo validation should be done.